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a, comparison of MUGS activity (nanomoles/mg of total cell protein/h) in the postnuclear supernatant (PNS) and lysosome-enriched (Lyso.) fraction from untreated (open bar) and NGT (0.9 mM) treated (filled bar) ATSD fibroblasts. b, Western blots comparing the levels of α-subunits of Hex (α Hex), mature β-subunits of Hex (β Hex), glucocerebrosidase (Gcase), and <t>calnexin</t> in the PNS and lysosomal fractions (Lyso.) from untreated and NGT-treated ATSD cells. Position of relevant Mr markers (in kDa) are shown to the left of the blots. One microgram of total protein from each of the PNS and lysosomal fractions were analyzed.
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a, comparison of MUGS activity (nanomoles/mg of total cell protein/h) in the postnuclear supernatant (PNS) and lysosome-enriched (Lyso.) fraction from untreated (open bar) and NGT (0.9 mM) treated (filled bar) ATSD fibroblasts. b, Western blots comparing the levels of α-subunits of Hex (α Hex), mature β-subunits of Hex (β Hex), glucocerebrosidase (Gcase), and <t>calnexin</t> in the PNS and lysosomal fractions (Lyso.) from untreated and NGT-treated ATSD cells. Position of relevant Mr markers (in kDa) are shown to the left of the blots. One microgram of total protein from each of the PNS and lysosomal fractions were analyzed.
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a, comparison of MUGS activity (nanomoles/mg of total cell protein/h) in the postnuclear supernatant (PNS) and lysosome-enriched (Lyso.) fraction from untreated (open bar) and NGT (0.9 mM) treated (filled bar) ATSD fibroblasts. b, Western blots comparing the levels of α-subunits of Hex (α Hex), mature β-subunits of Hex (β Hex), glucocerebrosidase (Gcase), and <t>calnexin</t> in the PNS and lysosomal fractions (Lyso.) from untreated and NGT-treated ATSD cells. Position of relevant Mr markers (in kDa) are shown to the left of the blots. One microgram of total protein from each of the PNS and lysosomal fractions were analyzed.
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Image Search Results


a, comparison of MUGS activity (nanomoles/mg of total cell protein/h) in the postnuclear supernatant (PNS) and lysosome-enriched (Lyso.) fraction from untreated (open bar) and NGT (0.9 mM) treated (filled bar) ATSD fibroblasts. b, Western blots comparing the levels of α-subunits of Hex (α Hex), mature β-subunits of Hex (β Hex), glucocerebrosidase (Gcase), and calnexin in the PNS and lysosomal fractions (Lyso.) from untreated and NGT-treated ATSD cells. Position of relevant Mr markers (in kDa) are shown to the left of the blots. One microgram of total protein from each of the PNS and lysosomal fractions were analyzed.

Journal:

Article Title: Pharmacological Enhancement of β -Hexosaminidase Activity in Fibroblasts from Adult Tay-Sachs and Sandhoff Patients *

doi: 10.1074/jbc.M308523200

Figure Lengend Snippet: a, comparison of MUGS activity (nanomoles/mg of total cell protein/h) in the postnuclear supernatant (PNS) and lysosome-enriched (Lyso.) fraction from untreated (open bar) and NGT (0.9 mM) treated (filled bar) ATSD fibroblasts. b, Western blots comparing the levels of α-subunits of Hex (α Hex), mature β-subunits of Hex (β Hex), glucocerebrosidase (Gcase), and calnexin in the PNS and lysosomal fractions (Lyso.) from untreated and NGT-treated ATSD cells. Position of relevant Mr markers (in kDa) are shown to the left of the blots. One microgram of total protein from each of the PNS and lysosomal fractions were analyzed.

Article Snippet: Donkey polyclonal antibody developed against a C-terminal calnexin peptide was purchased from Santa Cruz Biotechnology.

Techniques: Activity Assay, Western Blot